Use cases and applications in biotechnology

Take a look at how OF2i® our other BRAVE innovations are in use in biotech applications.

Early detection of protein aggregation in biopharmaceuticals e.g. monoclonal antibodies (mAbs)

BRAVE Analytics’ nanoparticle analyzer and online particle sensor provide a way to determine when aggregation starts to occur. It monitors the progression of aggregation as it happens via OF2i® and static light scattering signals[1] and delivers results in real-time, eliminating the delay inherent in waiting for offline measurements.

Reference: [1] Imaging the scattered light of a nanoparticle through a cylindrical capillary. Ulrich Hohenester, Christian Neuper, Marko Šimić, and Christian Hill. Nanophotonics, 13(4):457–463, 2024.

Understanding dynamic liquid-liquid phase separation (LLPS) processes by observing the changes in size and distribution of proteins over time

These time-resolved measurements on different concentrations of proteins and RNA reveal the kinetics of LLPS processes and allow deeper insights than ever before.

Investigating the aggregation of biomacromolecules (low-density liproteins, LDL) by combining OF2i® and SEC

The particle concentration of the fractions passing through the SEC column were continuously measured using the OF2i® static light scattering module. The results give insight into the properties of aggregated LDL versus non-aggregated LDL and also flagged which fractions where the purest and therefore best-suited for e.g. cryo-EM investigations.

Identifying and sizing low-density liproteins (LDL) with OF2i® and Raman spectroscopy

Building on the above experiments, we used Raman spectroscopy to distinguish between single LDL particles and LDL agglomerates and give a size range for LDL particles.

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Als Partner des NanoPAT Projekts hat BRAVE Analytics Mittel aus dem Forschungs- und Innovationsprogramm HORIZON 2020 der Europäischen Union unter der Fördervereinbarung Nr. 862583 erhalten.

Als Partner des MOZART project (MOZART for safe & sustainable by-design metallic coatings & engineered surfaces (RIA)) haben wir Mittel aus dem Programm HORIZON 2020 der Europäischen Union erhalten.

Use cases and applications in biotechnology

Inhaltsverzeichnis

Early detection of protein aggregation in biopharmaceuticals e.g. monoclonal antibodies (mAbs)

Understanding dynamic liquid-liquid phase separation (LLPS) processes by observing the changes in size and distribution of proteins over time

Investigating the aggregation of biomacromolecules (low-density liproteins, LDL) by combining OF2i® and SEC

Identifying and sizing low-density liproteins (LDL) with OF2i® and Raman spectroscopy